Chloroplast DNA digests

Michael Witty mw132 at mole.bio.cam.ac.uk
Sat Apr 27 20:27:22 EST 2002


On Fri, 26 Apr 2002, Johan Burger wrote:

> Dear All
>
> We extracted chloroplast DNA from Grapevine which we want to digest with
> different restriction enzymes. This seem to be a major problem, the DNA
> seems very good quality (judging from an agarose gel) but for some or other
> reason just won't cut. We tried several enzymes but everytime seem to get
> partial cuts at best.
>
> Does anyone know of any secrets or tricks that one should use to get
> Chloroplast DNA to digest properly?

Hi Johan,
        how did you purify your DNA and which enzymes are you using?
Mike.




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