T4 DNA Ligase

Peter Ianakiev ianakiev at genome.wi.mit.edu
Mon Apr 29 11:33:53 EST 2002


Hi guys

I have a problem with T4 DNA Ligase from NEB. It seems that it is
working even without rATP.
I used 1kb Ladder from NEB, incubated in 1x NEB-2 buffer and 400 NEB
Units Ligase for 24 h, and I was aiming to test the ligase prep for
nuclease activity. Surprisingly, it ligated my ladder without rATP!?
(NEB claims that there are no rATP in ligase storage buffer)
What this activity might be? RNA - ligase?
Any suggestions are greatly appreciated

Thank you

Peter




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