T4 DNA Ligase

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Apr 29 15:39:16 EST 2002


On Mon, 29 Apr 2002, Peter Ianakiev wrote:

> Hi guys
>
> I have a problem with T4 DNA Ligase from NEB. It seems that it is
> working even without rATP.
> I used 1kb Ladder from NEB, incubated in 1x NEB-2 buffer and 400 NEB
> Units Ligase for 24 h, and I was aiming to test the ligase prep for
> nuclease activity. Surprisingly, it ligated my ladder without rATP!?
> (NEB claims that there are no rATP in ligase storage buffer)
> What this activity might be? RNA - ligase?
> Any suggestions are greatly appreciated
>
> Thank you
>
> Peter

Did you contact NEB via their helpline?  They might own up to something.
Mike.




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