fluorescent dye primer extension

Frauke Naumann fnaumann at scan.genetik.uni-koeln.de
Thu Aug 1 12:20:04 EST 2002


dear all,
has anyone seen a reference in which the fluorescence labeled primers were
used for mapping of the transcriptional start site using ABI Prism 377? Is
fluorescence sensitive enough for a low expressed gene? Is FAM still the
most senstive dye and is it also *easy* to optimize?

thank you in advance

Frauke





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