NP40 inactivation of vaccinia virus

Ian A. York iayork at panix.com
Tue Aug 6 05:45:56 EST 2002


Does anyone know whether 1% NP40 or triton X-100 will inactivate vaccinia
virus?  (We need to run an enzyme assay in cells infected with recombinant
vaccinia; the enzyme is stable in NP40, and if we can eliminate infectious
virus it will be much less work to run the asssays.)  I assume that,
vaccinia being enveloped, the detergent will inactivate it, but I'd like  
to be sure.

Ian

-- 
    Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
    "-but as he was a York, I am rather inclined to suppose him a
     very respectable Man." -Jane Austen, The History of England



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