PAGE of DNA: EtBr

[Steve at genedetect.com]

Hi Peter

No it is not entirey neccessary...you can see bands almost within 5 minutes of de-staining. We just finish our gels at the end of the day and find overnight washing in water leaves the background staining almost non-existant and the bands are still dark.

So, yes, within 30 minutes of de-staining you can see bands very clearly.....overnight de-staining just increases the contrast between bands and background staining further.

Regards
Steve

www.genedetect.com
"World's largest selection of gene probes"


Peter Cherepanov wrote:

> I never tried methylene blue, 

> Is it necessary to destain your gel that long? 
> can you do the whole thing in half an hour?

> (agarose preparative gels, for example) 

> Peter

> ---





http://biowww.net/mynews/tree.php?group_name=bionet.molbio.methds-reagnts&begin=0