Antibody purification

Vayuputra vayuputra369 at hotmail.com
Fri Aug 9 06:15:22 EST 2002


Thanks again!

V


D.K. <dk at no.email.thankstospam.net> wrote in message
news:aivhjt$eef$1 at news.doit.wisc.edu...
> Glutathione is a tripeptide. Thus, it will naturally interfere with every
> protein immobilization chemistry except the ones that employ linkage
> through sugar modifications. With 20 mM in elution being typical, NH2
> concentration is going to exceed activated group concentration
> (10 umol/ml=10 mM is typical). Yes, a thorough, thorough dialysis
> against a strong buffer (you get acid as reaction product) that
> does not contain amines is essential.
>
> DK





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