Antibody purification

Vayuputra vayuputra369 at
Fri Aug 9 06:15:22 EST 2002

Thanks again!


D.K. <dk at> wrote in message
news:aivhjt$eef$1 at
> Glutathione is a tripeptide. Thus, it will naturally interfere with every
> protein immobilization chemistry except the ones that employ linkage
> through sugar modifications. With 20 mM in elution being typical, NH2
> concentration is going to exceed activated group concentration
> (10 umol/ml=10 mM is typical). Yes, a thorough, thorough dialysis
> against a strong buffer (you get acid as reaction product) that
> does not contain amines is essential.
> DK

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