alternatives to southern or FISH?

Volker Budinger budinger at uni-duesseldorf.de
Fri Aug 9 08:10:24 EST 2002


Hi,

I am characterising some cell lines which were transfected with a
linearised plasmid. Now i'm searching for a way to find out, how often
this single sequence was inserted into the genome of those HeLa's.

Probably this could be done by southern or FISH but I'am searching for
other alternative methods instead of these. (Cause I've got problems
with the sensitivity of the southern assay and fish could not be done in
our laboratory.)

Does anyone know alternative methods?

Thanks
Volker Budinger


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