alternatives to southern or FISH?

[Eric R. Hugo]

PCR of all are part of your sequence should be more than sensitive 
to detect your sequence.
Eric

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Eric R. Hugo, Ph.D.//   |:,\\': | \\ //   :,\\': | \\  
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budinger at uni-duesseldorf.de (Volker Budinger) wrote in 
news:3D5402DA.D4B3A625 at uni-duesseldorf.de:

> Hi,
> 
> I am characterising some cell lines which were transfected with a
> linearised plasmid. Now i'm searching for a way to find out, how often
> this single sequence was inserted into the genome of those HeLa's.
> 
> Probably this could be done by southern or FISH but I'am searching for
> other alternative methods instead of these. (Cause I've got problems
> with the sensitivity of the southern assay and fish could not be done in
> our laboratory.)
> 
> Does anyone know alternative methods?
> 
> Thanks
> Volker Budinger
> 
> 
> ---