Has anyone used terminal transferase to poly rA an in-vitro transcript? Thanks. Martyn Donnison

[Nick Theodorakis]

On 13 Aug 2002 02:38:17 +0100, martyn.donnison at agresearch.co.nz
("Donnison, Martyn") wrote:


> Has anyone used terminal transferase to poly rA an in-vitro transcript? Thanks. Martyn Donnison

[...]

No. But poly (A)polymerase works well enough. Or you can use TT to add
poly(dA) and poly(dT) to the template (and reclone it). I've done it
both ways.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu