help with DNA extraction
roland.hubner at ua.ac.be
Tue Aug 13 03:45:00 EST 2002
>>Sometimes we'll get a group of tissue samples (fin clips from fish)
>>that are faded out (the normal pigment looks washed out), and our
>>extracted product will be an oily brown droplet that will not
>> are you working with plants? In plants, the viscosity you mention is
>> often a result of large amounts of polysaccharides co-precipitating
>> with the DNA.
>We are extracting human tissue (prostate and kidney) which is taken at
>time of operation and frozen at -80 degrees until extraction. However,
>your oily brown droplet which looks OK on agarose but will not amplify
>- that sounds exactly the same as the extraction product I am now
could this be caused by blood-soaken samples?
(hemoglobin is emblematic PCR-inhibitor)
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