cloning ?

nazarian at nazarian at
Wed Aug 14 04:07:52 EST 2002

Hello everyone!
Is there anybody who have some experience from cloning
of binding domain botulinum neurotoxin. I am trying to amplify and clone and sequence it.PCR with pfu or taq is good and digestion of vector PET28a and PCR product is too.but in transformation ,I dont see clone of gene .please help me
Any help would bemuch appreciated.

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