Extraction of large DNA fragments from agarose gels

Dirk Müller Kraweel at web.de
Wed Aug 14 07:20:34 EST 2002


On Tue, 13 Aug 2002 17:33:13 +0200, "Johan Burger"
<jtb at maties.sun.ac.za> wrote:

>Hi All
>
>Can someone please tell me which is the best method(s) to extract 10-15 kb
>DNA fragments from agarose gels. Are there any commercial kits that can do
>this? What kind of yield can one expect?
>
>Thanks
>Johan Burger
>

We use for DNA fragments of your desired size the Quiagen PCR
purification kit with buffer QG (i think it is QG, may be you need to
by it seperate) for gelelution. Yield is from 50 % to 90 % .
Dirk...



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