Reasons for gel samples running unevenly?

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Aug 14 12:28:50 EST 2002


On 14 Aug 2002, Paul Wary wrote:

> What reasons can there be for gel samples running unevenly?
> For example, the same DNA marker on the left of my gel runs
> differently on the right, i.e. the corresponding bands are not exactly
> at the same places.
> With many samples next to each other I can also see it by the
> bromophenol blue band, which is not a straight line as it is supposed
> to be.
>
> Paul
>

. . . different salt content in different wells?

or a bubble under the gel (causeing local heating)?

or non-level apparatus, causing higher current flux in the "shallow" end?

Mike.




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