Extraction of large DNA fragments from agarose gels
Jose I. de las Heras
jose at hgu.mrc.ac.uk
Thu Aug 15 11:53:40 EST 2002
Johan Burger wrote:
> Hi All
> Can someone please tell me which is the best method(s) to extract 10-15 kb
> DNA fragments from agarose gels. Are there any commercial kits that can do
> this? What kind of yield can one expect?
> Johan Burger
I use Roche's Agarase in conjunction with low melting point agarose to
extract fragments of around 100Kb... no problems. Give it a try...
Dr. Jose I. de las Heras Email: Jose.delasHeras at hgu.mrc.ac.uk
MRC Human Genetics Unit Tel: (0131) 3322471
Chromosome Biology Section ext: 2306 (office)
Western General Hospital 2310 (lab)
Edinburgh EH4 2XU Fax: (0131) 3432620
Scotland - United Kingdom
"Musha ring dum a doo dum a da, Whack for my daddy-o"
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