pRSET expression system troubles

Wolfgang Schechinger marsupilamiwolfgang at web.de
Mon Aug 19 13:57:21 EST 2002


Joe, 
I have been using pRSET B. Worked fine with balb/3t3 and 293 HEK cells
(transfection with PEI and Lipofectamine). 

Could you give me some more details on your experimnent (promoter,
resistance, cells, insert, history)?

Regards,
Wo

At 13:33 19.08.02 -0400, you wrote:
>Hello all,
>
>I was wondering if anyone here has any experience expressing proteins using
>Invitrogen's pRSET system.  I am attempting to use it but so far it does not
>appear that my proteins are being manufactured.  If anybody else has
>experienced troubles similar to this with this system and could give me some
>suggestions about how to fix them I would be very greatful.
>
>Thanks,
>Alex Borrelli
>
>
>

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