Is this error forgivable?
khatipovNO at NOuchicago.edu
Sat Aug 24 19:31:08 EST 2002
Some labs even use 250mg/ml Amp, so don't worry. If I am not mistaken, Amp
decomposes at 37C by ~50% over a 36hr peroid anyway, even without cells
growing on a plate.
"JSUPolek" <jsupolek at aol.com> wrote in message
news:20020824081117.12380.00003288 at mb-fm.aol.com...
> Added 2X the concentration of ampicillin to my LB agar that I was supposed
> (100ug/ml v. 50 ug/ml). Using DH5-alpha and pGEM. Any reason not to use
> Let them incubate a little longer? Thanks
More information about the Methods