Is this error forgivable?

Michael Witty mw132 at mole.bio.cam.ac.uk
Sat Aug 24 20:30:25 EST 2002


On Sat, 24 Aug 2002, Emir Khatipov wrote:

> Some labs even use 250mg/ml Amp, so don't worry. If I am not mistaken, Amp
> decomposes at 37C by ~50% over a 36hr peroid anyway, even without cells
> growing on a plate.
> Emir

. . . I was surprised by how long amp lasts in agar at 4 degrees.  I have
been using amp plates that have almost (but not quite) dried out
completely to transform E. coli.  They could soak up 1ml of LB per plate.
Worked really well.  Regards, Mike.




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