mgarbutt at mb.sympatico.ca
Mon Aug 26 19:50:17 EST 2002
> I have 135 bp cDNA probe that I would like to label with non radioactive
> method but I don not know the best way to do it?. I'm planing to use this
> probe to screen bacterial colonies (mamalian BAC subclones) and for southern
> analysis. I used a kit from Ambion called BrightStar kit, but I got lot of
> background and could not confirm what I thought positive clones with southern
Amersham has a HRP conjugate kit. It takes about 10 min to label the
probe, 15 min to 1 h to block, then you can get right on with labeling.
The blocking solution takes less than one hour to prepare (mostly
rocking time). Very fast... very effective.
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