pRSET expression system troubles
Kyle Legate
legatek at mcmail.cis.mcmaster.ca
Tue Aug 27 15:50:09 EST 2002
On Sun, 25 Aug 2002, Joe Bagshaw wrote:
> This protein is bacteriacidal but it functions by coming together in groups
> and forming unregulated pores. I am thinking that even though it is toxic
> the massive his/x-press tag complex will hinder its activity.
>
You might be SOL if the tag doesn't hinder activity. Does this protein
form pores in yeast? Perhaps they would be a better overexpresser.
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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