pRSET expression system troubles

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Tue Aug 27 15:50:09 EST 2002


On Sun, 25 Aug 2002, Joe Bagshaw wrote:

> This protein is bacteriacidal but it functions by coming together in groups
> and forming unregulated pores.  I am thinking that even though it is toxic
> the massive his/x-press tag complex will hinder its activity.
>
You might be SOL if the tag doesn't hinder activity. Does this protein
form pores in yeast? Perhaps they would be a better overexpresser.

... . . .  .  .  .    .    .    .     .     .     .      .      .      .
legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

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