Western Blot Smearing?

oswaldo oswaldo at REMOVETHISiats.csic.es
Wed Aug 28 11:55:43 EST 2002


it could be a blocking problem, how are you doing it?. Have you run any 
control lane without primary PAb or with a pre-immunization rabit serum?

oswaldo




d.white at genesis.co.nz wrote:
> I'm currently having recurrent problems with western blot analysis, specifically with excessive smearing around the top of the blot, and frustration is setting in.
> 




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