help about GST-fusion protein SDS-PAGE

fly1234 at yeah.net fly1234 at yeah.net
Thu Aug 29 06:34:27 EST 2002


I expressed a GST-fusion protein in BL21 and purifid it with glutathione beads. After SDS-PAGE, I was surprised to find that the apparent Molecular weight of the purified product (in 20mm glutatione buffer) was several KD larger than that of pre-purified target protein (showed as a bold band) in cell lysate. Does anybody have the same experience? Could you help me?




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