are all 3 stop codons equally efficient

Josmar Langner jlangner at
Thu Aug 29 07:55:56 EST 2002

Hi there,
I'm wondering, if all 3 stop codons are equally efficient in E.coli.
If I want to disrupt an ORF, which would be the best stop codon to
introduce. What about repeats of stop codons. I'm well aware of the
fact, not to use bacterial strains, that have suppressor t-RNAs.

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