help about GST-fusion protein SDS-PAGE

Iva Toudjarska toudjarska at wi.mit.edu
Fri Aug 30 09:15:27 EST 2002


If you want to see quickly if glycosilation is the cause, Pierce (no affiliation) cells glycoprotein staining kit

good luck
Iva

fly1234 at yeah.net wrote:

> I expressed a GST-fusion protein in BL21 and purifid it with glutathione beads. After SDS-PAGE, I was surprised to find that the apparent Molecular weight of the purified product (in 20mm glutatione buffer) was several KD larger than that of pre-purified target protein (showed as a bold band) in cell lysate. Does anybody have the same experience? Could you help me?
>
> http://biowww.net/mynews/tree.php?group_name=bionet_molbio_methds-reagnts&begin=0
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