help about GST-fusion protein SDS-PAGE
toudjarska at wi.mit.edu
Fri Aug 30 09:15:27 EST 2002
If you want to see quickly if glycosilation is the cause, Pierce (no affiliation) cells glycoprotein staining kit
fly1234 at yeah.net wrote:
> I expressed a GST-fusion protein in BL21 and purifid it with glutathione beads. After SDS-PAGE, I was surprised to find that the apparent Molecular weight of the purified product (in 20mm glutatione buffer) was several KD larger than that of pre-purified target protein (showed as a bold band) in cell lysate. Does anybody have the same experience? Could you help me?
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Methods