Freezing cultured cells

Tom Anderson univ0938 at herald.ox.ac.uk
Wed Dec 4 13:24:52 EST 2002


On Thu, 5 Dec 2002, Oncar wrote:

> Someone teach me to freeze cultured cells by 10%DMSO in FBS instead of
> 10% DMSO in culture medium and says cells will be in excellent
> condition, i would like to know is there anyone do so? and what's yours
> method indeed ?

we do exactly that - 10% DMSO in FCS. it works fine.

tom

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