Primer design

Chang Zhu czhu at changbioscience.com
Wed Dec 4 16:27:53 EST 2002


Try Primo Pro:

http://www.changbioscience.com/primo/

I would really appreciate if someone could give me some feedbacks.

Chang

Tom Anderson <univ0938 at herald.ox.ac.uk> wrote in message news:<Pine.OSF.4.44.0212021322510.553-100000 at ermine.ox.ac.uk>...
> On Fri, 29 Nov 2002, Phoenix wrote:
> 
> > I would like to know the succcessful rate of the primers designed by primer
> > 3 in PCR?
> 
> i haven't actually tried my big primer3 primers yet. for the applications
> for which it was intended (STS analysis and the like), i think it's
> generally quite successful.
> 
> > Coz i usually fail in PCR if i decide primer by myself and need to order
> > several pairs of primers : (
> 
> primer design really is a bit of a black art - if you follow the rules
> about length, melting temperature, having a few C/G bases at the 3' end,
> etc, it'll usually work, but sometimes, for no apparent reason, it
> doesn't.
> 
> tom



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