Maximum distance between ATG & SP6 promoter

[Trond Erik Vee Aune]

Chang Zhu wrote:
> What should be the optimal distance between Shine-Dalgarno (SD) and the 
> AUG? 

First of all, the Shine-Dalgarno is not a part of the promotor, it's a 
part of the translation initiation region (TIR).

The optimal distance between the start codon and the SD differs from 
strain to strain. In E.coli it is 8-12.

Trond Erik