RIbosome isolation

[oustudygirl at hotmail.com]

I need to isolate whole, intact and functional ribosomes from plant leaf tissue, while diminishing RuBisco or removing chloroplast. All the methods I have found were either to isolate whole mammalian ribosomes, or isolate ribosomal subunits in plants. I`ve been trying to combine and create an adequate method but I get very low yeilds or RuBisco contamination. Can any helpÉ


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