exclusion threshold for PCR purification

denis dupuy denis_dupuy at dfci.harvard.edu
Mon Dec 16 15:41:55 EST 2002

Hello everyone,

I'm involved in a large scale PCR cloning project and I have trouble
with contaminant bands that are about 100bp (which are primers dimers).

I'm looking for a 96-well format protocol that would allow PCR
purification of my genuine PCR product.

All the usual kits are set up to retrieve PCR products as small as

Does anybody know a way to modify this cuttoff so that I could retrieve
only bigger fragments and get rid of my contaminant 100bp-species?

Thank you in advance


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