exclusion threshold for PCR purification

denis dupuy denis_dupuy at dfci.harvard.edu
Mon Dec 16 15:41:55 EST 2002


Hello everyone,

I'm involved in a large scale PCR cloning project and I have trouble
with contaminant bands that are about 100bp (which are primers dimers).

I'm looking for a 96-well format protocol that would allow PCR
purification of my genuine PCR product.

All the usual kits are set up to retrieve PCR products as small as
100bp.

Does anybody know a way to modify this cuttoff so that I could retrieve
only bigger fragments and get rid of my contaminant 100bp-species?

Thank you in advance

Denis




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