Ligation of short DNA fragments.

Michael Witty mw132 at mole.bio.cam.ac.uk
Tue Dec 17 11:43:43 EST 2002


On Mon, 16 Dec 2002, Zhongtang wrote:

> I want to construc concatemers of 40-bp dsDNA fragments by T4 ligation.

. . . why not redesign your primers and make a system of longer
overlapping ones and then use a PCR reaction with Taq polymerase?  No need
to worry about phosphorylation then.  Regards, Mike.




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