LightCycler quantitation inconsistencies

hospital_scientist hospital_scientist at yahoo.com.au
Wed Dec 18 02:13:49 EST 2002


Has anyone had any problems with inconsistant results using the Roche
LightCycler for quantitation? I read an article in Biotechniques
recently that related primer melting temps to quantitation errors
(someone has taken my copy of biotechniques and not returned it). This
may explain some glitches I experienced while trying to develop some
viral detection assays.

Sincerely,

A virologist in Australia...



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