exclusion threshold for PCR purification

Duncan Clark junk at hgmp.mrc.ac.uk
Wed Dec 18 06:27:00 EST 2002

Historians believe that in newspost <3DFE3A92.7EB62F5F at dfci.harvard.edu> 
on Mon, 16 Dec 2002, denis dupuy <denis_dupuy at dfci.harvard.edu> penned 
the following literary masterpiece:
>I'm involved in a large scale PCR cloning project and I have trouble
>with contaminant bands that are about 100bp (which are primers dimers).

Hot Start, reduce primer concns, increase the annealing temp - gradient 
blocks are nice for this, optimise Mg etc etc

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

More information about the Methods mailing list