Help, my negative control is positive

Kyle Legate legatek at mcmail.cis.mcmaster.ca
Wed Dec 18 10:18:41 EST 2002


On 18 Dec 2002, Peter wrote:

> The recombinant protein I use is a human protein. After first boosting
> with the recombinant prame, i boosted the rabbit after a while with
> peptides from this protein. In an ELISA assay the peptides are
> recognized very strong, the recombinant protein also. an other
> recombinant protein wich is made the same way is not recognized.
>
Solution: try again. Try two different rabbits to double your chances of
success.

... . . .  .  .  .    .    .    .     .     .     .      .      .      .
legatek at mcmaster.ca		Kyle Legate            legatek at hotmail.com

   Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
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