Hot Start PCR failures

Duncan Clark junk at hgmp.mrc.ac.uk
Sat Dec 21 05:12:29 EST 2002


Historians believe that in newspost 
<646eb82d.0212191520.68f0dda7 at posting.google.com> on Thu, 19 Dec 2002, 
James Prescott <prescj at dianon.com> penned the following literary 
masterpiece:
>Does anyone have any evidence of wax inhibiting the PCR or
>is it most likely a mixing problem?

Many additives in wax which can interfere with PCR. Makes the wax better 
for what it is used for but not so good for PCR.

Why not add Taq antibody to the Expand HIfi mix? The proof-reading 
component has minimal activity at RT whilst the AB will knock out the 
Taq component. Buffer stays the same.

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.



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