pEGFP-C1 transformation

Duncan Clark junk at
Mon Dec 23 09:12:49 EST 2002

Historians believe that in newspost <au47e6$kkd$1 at> 
on Sun, 22 Dec 2002, Carnac <carnac at> penned the 
following literary masterpiece:
>Ah, this is interesting... I didn't actually heat kill the ligase.  Do you
>think this is the most likely possibility for the poor transformations?

There was something published as a single sheet in the back of NAR, 
maybe 4-5 years or so ago. Heat killing was the key to good 
electroporation, plus of course dropping the salts. Heat killing can't 
do any harm so worth a go.

I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.

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