pEGFP-C1 transformation

Carnac carnac at realitys-edge.com
Mon Dec 23 17:44:05 EST 2002


"EK" <khatipovNO-SPAM at NO-SPAMuchicago.edu> wrote in message
news:oYIN9.37$w4.3636 at news.uchicago.edu...
> pEGFP-C1 (Clontech, encoding EGFP with C-terminal His6 tag) encodes for
> beta-lactamase (ampicillin resistance). I wonder why even a few colonies
> survived on the kanamycin plates. Probably your E.coli stocks are not
pure,
> or you flame-dried your plates for too long :-)
> Emir

Na, it's definitely got a kanamycin resistance gene...






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