help on DNA sequence problem,anxious

shelley025 at shelley025 at
Mon Dec 30 12:59:46 EST 2002

Help on DNA sequencing:urgently

Use T7 primer, big dye terminator chemistry. However the signal stops at ~300BP. Maybe there is secondary structure lies in that region.  I tried several ways to solve this problem. Adding DMSO, increasing denature temperature, using different primers. But it still does not work.
Who met this same problem before? Can you give me some suggestion?

Thank you very very much!!

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