help on DNA sequence problem,anxious

[shelley025 at yahoo.com]

Help on DNA sequencing:urgently

Use T7 primer, big dye terminator chemistry. However the signal stops at ~300BP. Maybe there is secondary structure lies in that region.  I tried several ways to solve this problem. Adding DMSO, increasing denature temperature, using different primers. But it still does not work.
Who met this same problem before? Can you give me some suggestion?

Thank you very very much!!   



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