help on DNA sequence problem,anxious
hubahopp at gmx.de
Mon Dec 30 16:18:43 EST 2002
Could it be a problem in the base caller software? What does the raw data
look like? If the problem sits in the sequencing, maybe adding some DMF
before electrophorsis might help.
At 05:59 PM 2002/12/30 -0000, shelley025 at yahoo.com wrote:
>Help on DNA sequencing:urgently
>Use T7 primer, big dye terminator chemistry. However the signal stops at
~300BP. Maybe there is secondary structure lies in that region. I tried
several ways to solve this problem. Adding DMSO, increasing denature
temperature, using different primers. But it still does not work.
>Who met this same problem before? Can you give me some suggestion?
>Thank you very very much!!
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