Specific gene deleteion

Mario Tello mcg_tello at hotmail.com
Tue Dec 31 14:52:05 EST 2002


Hi everybody, I'm traiting to delete a gene by the method described by
Datsenko and Wanner, 2000. However this gene is part of a 13 Kpb
region previously cloned in a ColE1, Ampicilin resistant plasmid. This
plasmid is not compatible (have the same resistence)  with the plasmid
pKD46 where is cloned  the Red recombinase. Any body colud say how can
I make this deletion?, or if does exist a construction able to express
the recombinase with a selectable marker different from the ampicilin
resistence?

sincerely yours

Mario Tello
PhD student



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