toudjarska at wi.mit.edu
Fri Feb 1 12:48:44 EST 2002
My recomendation is try PureScript RNA isolation Kit from Gentra. It does not
contain phenol, it's fast and I have used it to isolate RNA for RPA - perfect
quality. With tissues you need to work fast and on ice. In my personal
experience just a regular agarose gel, in 1x TAE with well washed
electrophoretic tank run as quickly as posible is perfect for visualisation of
For more info on this kits www.gentra.com
O KANSAS wrote:
> I am trying to separate total RNA from some mouse tissues
> such as liver and kidney. I have used TriReagent (Sigma) and
> acid-guanidine isothiocyanate protocols. I have got nice looking 18S
> and 28S bands and above 1.8 A260/280 ratio. But the problem is
> abnormally stong 5S/7S rRNA-like band in the gel (Mops and
> formaldehyde). Normally, 5S/7S rRNA bands are much weaker than 18S or 28S
> rRNAs. I thought this is DNA contaminant or salt at first, but the strong
> band (and 18s/28S) could be removed after RNase treatment. I would like to
> remove this band for my next procedure (RNase protection).
> Any suggestion?
> Thank you.
> O Kansas
> opkansas at hananet.net
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