Baculovirus expression systems - which one??
anthea.scothern at umist.ac.uk
Mon Feb 4 11:29:58 EST 2002
I am trying to decide between the Bac-to-Bac from what was Gibco, BacnBlue
from Invitrogen (both now available in the Invitrogen catalogue), Clontech's
Bac Pak and Novagen's BacVector system - after a day of reading catalogues,
I can't handle any more BacAnything.
Does anyone have any recent experience with any of these kits/vectors/viral
DNA? My archive search only shows discussions a couple of years old.
I've been using an inherited combination of Baculogold DNA with BacPak
vectors and my virus stocks are very unreliable - sometimes expression,
sometimes not; so I think it's time to reassess and reclone. Any comments
would be gratefully received.
More information about the Methods