ETBr in Loading Buffer
David L Haviland
David.L.Haviland at uth.tmc.edu
Tue Feb 5 09:23:08 EST 2002
> I was wondering if anyone has ever tried or knows why Ethidium Bromide
> is not added to Loading buffer? I've never run across an article where
> Ethidium Bromide is present in the Loading Buffer, it's allways
> present either in the gel or as a post electorphoresis step.
ALL THE TIME... I put EtBr in the gel (2-4ul/100mls) and I have it in
my loading buffer at 1ug/ml. I never use it in the running buffer as
we can't pour the buffer down the drain otherwise. It has to be
filtered through a EtBr reclaimation filter. I've never had a problem
in doing this over the last 10 years, neither in the running of nor in
the interpretation of the data. In fact, it was even a suggestion out
of BioTechniques, a millenia ago... I can't even remember the issue.
I even do it for northerns too but drop the amount in the gel to 1-
2ul/100mls of gel, lest the formaldehyde turn the gel into a "pink
flare" on the transilluminator.
David L. Haviland, Ph.D., Asst. Prof. Immunology
University of Texas - Houston, H.S.C.
Institute of Molecular Medicine, R907
2121 W. Holcombe Blvd., Houston, TX 77030
If everything seems to be going so well, you have obviously
More information about the Methods