in vivo luciferase assay

Bertrand Collet b.collet at abdn.ac.uk
Wed Feb 6 10:36:34 EST 2002


Dear all,

I am using luciferase system (stable cell line expressing a promoter-luc
construct) in a destructive way (cell lysis + luminometer). Is there a
way to use it like GFP, i.e. incubate with luciferine without killing
the cells and take a photo in a darkroom ? Would it be sensitive enough
to get the glowing cells photographed ?
Thanks for any help/input,

Bertrand

-- 
Dr Bertrand Collet
Fish immunology group
Zoology Department
Tillydrone avenue
Aberdeen AB24 2TZ
Scotland - UK

Tel (office): (+44)(0)(1224)27 3796
Tel (lab): (+44)(0)(1224)27 2870
Fax: (+44)(0)(1224)27 2396




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