ETBr in Loading Buffer

Sergio seraln at _NO_SPAM_netscape.net
Wed Feb 6 10:08:00 EST 2002



David L Haviland wrote:

>>I was wondering if anyone has ever tried or knows why Ethidium Bromide
>>is not added to Loading buffer? I've never run across an article where
>>Ethidium Bromide is present in the Loading Buffer, it's allways
>>present either in the gel or as a post electorphoresis step.
>>
> 
> Hi... 
> 
> ALL THE TIME... I put EtBr in the gel (2-4ul/100mls) and I have it in 
> my loading buffer at 1ug/ml.  I never use it in the running buffer as 
> we can't pour the buffer down the drain otherwise.  


Watch out, even if you add the EtBr in the gel only, it moves out from gel to 
the running buffer due to it migrates to the anode. You shoul check your running 
buffer before pouring it down.

Sergio






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