ETBr in Loading Buffer

Henk Veldman H.Veldman-2 at
Thu Feb 7 02:32:32 EST 2002

Sergio schreef:

> David L Haviland wrote:
> >>I was wondering if anyone has ever tried or knows why Ethidium Bromide
> >>is not added to Loading buffer? I've never run across an article where
> >>Ethidium Bromide is present in the Loading Buffer, it's allways
> >>present either in the gel or as a post electorphoresis step.
> >>
> >
> > Hi...
> >
> > ALL THE TIME... I put EtBr in the gel (2-4ul/100mls) and I have it in
> > my loading buffer at 1ug/ml.  I never use it in the running buffer as
> > we can't pour the buffer down the drain otherwise.
> Watch out, even if you add the EtBr in the gel only, it moves out from gel to
> the running buffer due to it migrates to the anode. You shoul check your running
> buffer before pouring it down.

You can safely pour your running buffer down the drain after removing the EtBr by
absorbing it on activated charcoal or, more conveniently, with adsorption bags
(Amresco) that can accumulate up to 5 mg EtBr, enough for many liters of buffer.

H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)

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