Salmonella conjugation

Sergio seraln at _NO_SPAM_netscape.net
Thu Feb 7 12:02:27 EST 2002



Rafael Maldonado wrote:

> Dear Tamsin,
> 
> Teh genotype of the Salmonella strain would be useful, so could select
> in minimal medium (i.e. without proline) and with the requeriments for
> the Salmonella strain. Other ways to do it are:
> 
> - Use a Salmonella selective medium, as Salmonella-Shigella agar (check
> a bacteriology manual).
> - Use a lytic phage to kill E. coli (P1)
> 
> Normally, Salmonela is lac- and E. coli lac+ (although most E. coli from
> the la are lac- mutants, you shlud check that). You may use minimal
> medium with lactose as carbon source or indicator medium with lactose as
> McConkey.


That sounds to be a good method. As far as i know, S17-1 is lacZ+...

Sergio.




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