Southern Problems

Laura lcrews at
Fri Feb 8 14:40:20 EST 2002

I have been trying to fine tune my Southern's for months with no luck.  I am
using genomic cotton DNA with 100 bp probes and a DIG labeling kit.  I have
hybridized my probe back to the DNA it was synthesized from as a control
with success.  I can't get my probe to hybridize to the genomic DNA.  I have
used loaded up to 80 micrograms of genomic DNA.  Does anyone have any tips? 


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