lcrews at wcrl.ars.usda.gov
Fri Feb 8 14:40:20 EST 2002
I have been trying to fine tune my Southern's for months with no luck. I am
using genomic cotton DNA with 100 bp probes and a DIG labeling kit. I have
hybridized my probe back to the DNA it was synthesized from as a control
with success. I can't get my probe to hybridize to the genomic DNA. I have
used loaded up to 80 micrograms of genomic DNA. Does anyone have any tips?
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