PCR Primer Design Question
Markus.Ruch at access.unizh.ch
Mon Feb 11 07:28:50 EST 2002
I'm not that used in primer design, maybe someone can help me: For designing
a primer pair for a microsatellite locus I only got two possibilities left
(cloned DNA is pretty short):
1. A primer pair without any GC 5' clamp
2. A pair with ...GG endings each, but one ends with ...CATTTTTGG 5'
So what's worse, a poly-T close to the end, or primers without any
GC-clamps? Are there any good links for such questions?
Thank you for any hint.
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