PCR Primer Design Question

Markus Ruch Markus.Ruch at access.unizh.ch
Mon Feb 11 07:28:50 EST 2002


Hi all

I'm not that used in primer design, maybe someone can help me: For designing
a primer pair for a microsatellite locus I only got two possibilities left
(cloned DNA is pretty short):
1.  A primer pair without any GC 5' clamp
2.  A pair with ...GG endings each, but one ends with ...CATTTTTGG 5'

So what's worse, a poly-T close to the end, or primers without any
GC-clamps? Are there any good links for such questions?

Thank you for any hint.
Sincerely
Markus




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