PCR Primer Design Question

Markus Ruch Markus.Ruch at access.unizh.ch
Mon Feb 11 07:39:06 EST 2002


Whoops, just displace the 5' with 3'...

Markus Ruch wrote:

> Hi all
>
> I'm not that used in primer design, maybe someone can help me: For designing
> a primer pair for a microsatellite locus I only got two possibilities left
> (cloned DNA is pretty short):
> 1.  A primer pair without any GC 5' clamp
> 2.  A pair with ...GG endings each, but one ends with ...CATTTTTGG 5'
>
> So what's worse, a poly-T close to the end, or primers without any
> GC-clamps? Are there any good links for such questions?
>
> Thank you for any hint.
> Sincerely
> Markus




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