blue/white screening

licchesi j.licchesi.1999 at cranfield.ac.uk
Tue Feb 12 10:09:02 EST 2002


Dear all

I am trying to clone pcr product (purified) into pgem t easy vector
(promega). While my positif control produce a lot of white colonies (with
insert) the transformation with the pcr product (around 500bp) only
generates blue colonies and blueish colonnies. After screening of those
colonnies no insert was found.
Any help is welcome

julien


<http://www.biowww.net/forum/read.php?f=1&i=5309&t=5309>




More information about the Methods mailing list